| 1 | Luisa María Gonzalez Salazar (Neo) | ESP | 39.08 | | - |
| 2 | Sara Cabrera (Senior) | ESP | 39.82 | PB | +0.740 |
| 3 | Ona Rodriguez Cornejo (B2) | ESP | 40.17 | | +1.090 |
| 4 | Jasmin Güntert (Senior) | SUI | 40.59 | SB | +1.510 |
| 5 | Hanna Biró (Neo) | HUN | 40.93 | | +1.850 |
| 6 | Mia Meinig (B2) | GER | 41.70 | PB | +2.620 |
| 7 | Dalia Soberanis (Senior) | GUA | 42.12 | SB | +3.040 |
| 8 | Franziska Riedel (A1) | GER | 42.63 | | +3.550 |
| 9 | Lucie Korvasova (Neo) | CZE | 42.73 | | +3.650 |
| 10 | Katerina Macháckova (Neo) | CZE | 43.09 | | +4.010 |
| 11 | Emma Schöler (B1) | GER | 43.40 | PB | +4.320 |
| 12 | Mika Louise Brandt (B1) | GER | 43.87 | | +4.790 |
| 13 | Silvia Federico (Senior) | ITA | 44.00 | | +4.920 |
| 14 | Evi Blomberg (C2) | NED | 44.43 | | +5.350 |
| 15 | Barbora Sábliková (C2) | CZE | 44.54 | | +5.460 |
| 16 | Janice Eloane Lamien (C1) | AUT | 44.56 | PB | +5.480 |
| 17 | Marta Papoušková (C2) | CZE | 44.60 | PB | +5.520 |
| 18 | Tami Altrogge (A2) | GER | 44.77 | SB | +5.690 |
| Hanna Hnatiuk (A2) | UKR | 44.77 | | +5.690 |
| 20 | Imre Blomberg (D2) | NED | 45.39 | | +6.310 |
| 21 | Anna Berczelly (B1) | AUT | 45.68 | PB | +6.600 |
| 22 | Johana Pracharova (A1) | CZE | 46.01 | PB | +6.930 |
| 23 | Lucie Pecová (C1) | CZE | 48.00 | PB | +8.920 |
| 24 | Anna Gaierová (A2) | CZE | 48.16 | PB | +9.080 |
| 25 | Martina Sedláčková (D2) | CZE | 48.68 | PB | +9.600 |
| 26 | Margaryta Lupekha (C2) | UKR | 49.01 | | +9.930 |
| 27 | Emily Lai (D2) | CZE | 49.29 | | +10.210 |
| 28 | Anna Zauner (D1) | GER | 50.89 | | +11.810 |
| 29 | Anděla Procházková (E1) | CZE | 51.47 | PB | +12.390 |
| 30 | Maryna Osypenko (D1) | UKR | 52.37 | | +13.290 |
| 31 | Marijn Zwaneveld (C1) | NED | 53.61 | | +14.530 |
| 32 | Amelia McCormack (Senior) | GBR | 55.94 | PB | +16.860 |
| 33 | Lucie Vašková (E1) | CZE | 55.99 | | +16.910 |
| 34 | Kathleen Toth (M45) | SUI | 57.19 | | +18.110 |
| 35 | Daria Ostrochereva (n/a) | UKR | 1:01.12 | | +22.040 |
| DNS | Elisa Spiegel (B2) | GER | DNS | | - |