| 1 | Lauren Teunissen (B1) | NED | 46.55 | | - |
| 2 | Manon van de Vliert-Scholte (M45) | NED | 48.93 | | +2.380 |
| 3 | Kristy Ton (C2) | NED | 49.70 | | +3.150 |
| 4 | Rikst van der Helm (Senior) | NED | 50.07 | SB | +3.520 |
| 5 | Marloes Heus (Senior) | NED | 50.24 | SB | +3.690 |
| 6 | Marre Looijen (B1) | NED | 50.53 | | +3.980 |
| 7 | Marit Kok (Senior) | NED | 50.85 | | +4.300 |
| 8 | Linda Eggenkamp (M50) | NED | 50.87 | | +4.320 |
| 9 | Annet de Riddder - Rijneveld (M30) | NED | 51.41 | SB | +4.860 |
| 10 | Ellemiek Gerritsen (B1) | NED | 53.25 | | +6.700 |
| 11 | Bianca Vermeulen (M35) | NED | 53.36 | SB | +6.810 |
| 12 | Dinte van Es (D2) | NED | 55.38 | | +8.830 |
| 13 | Ilse Baltussen (M40) | NED | 58.03 | SB | +11.480 |
| 14 | Fenne Looijen (D2) | NED | 59.92 | | +13.370 |
| 15 | Marjolijn Theunissen (M55) | NED | 1:00.60 | SB | +14.050 |
| 16 | Maaike Stuivenberg (C1) | NED | 1:01.95 | | +15.400 |
| 17 | Bebette Bikker (D2) | NED | 1:04.00 | SB | +17.450 |
| 18 | Astrid Robat (M50) | NED | 1:04.77 | SB | +18.220 |
| 19 | Eva van Muijden (D2) | NED | 1:04.96 | PB | +18.410 |
| 20 | Harmen Looijen (D2) | NED | 1:11.41 | | +24.860 |