| 1 | Leeyen Harteveld (C1) | NED | 44.18 | | - |
| 2 | Dewy Harteveld (B1) | NED | 44.81 | | +0.630 |
| 3 | Charlotte Kors (B1) | NED | 45.45 | PB | +1.270 |
| 4 | Sophie Moerman (Neo) | NED | 45.58 | | +1.400 |
| 5 | Elsemieke van Zuylen (B1) | NED | 46.78 | PB | +2.600 |
| 6 | Liesbeth Veen-Milatz (B1) | NED | 47.19 | SB | +3.010 |
| 7 | Claudia Oranje (B1) | NED | 47.84 | SB | +3.660 |
| 8 | Patricia van der Kooy (B2) | NED | 47.95 | SB | +3.770 |
| 9 | Merith van 't Hoff (B1) | NED | 48.55 | | +4.370 |
| 10 | Michelle de Bruijn (C2) | NED | 48.80 | | +4.620 |
| 11 | Kira Abbenhuijs (C1) | NED | 48.91 | PB | +4.730 |
| 12 | Marianne de Neeling (M45) | NED | 49.01 | | +4.830 |
| 13 | Evelien van Tol (B2) | NED | 49.14 | | +4.960 |
| 14 | Fieke van der Helm (B2) | NED | 49.18 | | +5.000 |
| 15 | Marloes Gravesteijn (Senior) | NED | 49.86 | | +5.680 |
| 16 | Joyce Buijs (C2) | NED | 50.65 | SB | +6.470 |
| 17 | Stacey Lansbergen (C1) | NED | 51.61 | | +7.430 |
| 18 | Netty de Bruijn (M40) | NED | 54.03 | | +9.850 |