| 1 | Chrysta Rands-Evans (Senior) | USA | 39.80 | | - |
| 2 | Sofia Prosvirnova (Senior) | RUS | 39.92 | SB | +0.120 |
| 3 | Maria Victoria Rodriguez (Senior) | ARG | 39.95 | | +0.150 |
| 4 | Anna Quinn (Neo) | USA | 40.69 | | +0.890 |
| 5 | Laura Michelle Rodriguez (B2) | COL | 41.08 | | +1.280 |
| 6 | Natalia Valencia (Senior) | COL | 42.01 | PB | +2.210 |
| 7 | Shruti Kotwal (M30) | IND | 42.21 | NRM | +2.410 |
| 8 | Ella Teeples (C1) | USA | 43.71 | | +3.910 |
| 9 | Isabela Vargas Rodriguez (B1) | COL | 44.02 | | +4.220 |
| 10 | Avery Hayes (C1) | USA | 45.26 | PB | +5.460 |
| 11 | Selena Su (C2) | USA | 45.39 | | +5.590 |
| 12 | Baily Luck (Neo) | USA | 51.81 | | +12.010 |