| 1 | Jekaterina Malysjeva (Neo) | RUS | 38.87 | | - |
| 2 | Elli Ochowicz (Senior) | USA | 39.01 | | +0.140 |
| 3 | Jennifer Rodriguez (Senior) | USA | 39.35 | | +0.480 |
| 4 | Heather Richardson (Neo) | USA | 39.65 | | +0.780 |
| 5 | Lauren Cholewinski (Neo) | USA | 40.03 | | +1.160 |
| 6 | Rebekah Bradford (Senior) | USA | 40.10 | | +1.230 |
| 7 | Ericka Hawke (Neo) | USA | 41.12 | | +2.250 |
| 8 | Kelly Gunther (Neo) | USA | 41.43 | | +2.560 |
| 9 | Catherine Raney-Norman (Senior) | USA | 41.60 | SB | +2.730 |
| 10 | Ashlee Barnett (Neo) | USA | 41.72 | | +2.850 |
| 11 | Mia Manganello (A2) | USA | 42.01 | | +3.140 |
| 12 | Netanya Mintz (Senior) | USA | 42.17 | | +3.300 |
| 13 | April Medley (Neo) | USA | 42.86 | | +3.990 |
| 14 | Anne Bruckner (Neo) | USA | 42.88 | | +4.010 |
| 15 | Sara Nielson (A2) | USA | 46.04 | | +7.170 |
| 16 | Liz Looby (A2) | USA | 47.10 | | +8.230 |
| 17 | Jamie Lindquist-Nielson (E1) | USA | 56.08 | | +17.210 |