| 1 | Haike Verbree (Neo) | NED | 43.31 | | - |
| 2 | Marieke Mulder (A1) | NED | 43.86 | | +0.550 |
| 3 | Nienke Kooistra (Senior) | NED | 44.39 | | +1.080 |
| 4 | Maike Kroes (Neo) | NED | 45.14 | SB | +1.830 |
| 5 | Jeike Juk (Neo) | NED | 45.69 | SB | +2.380 |
| 6 | Jinke Miriam van Keulen (Neo) | NED | 45.87 | SB | +2.560 |
| 7 | Tessa Kleinhuis (Neo) | NED | 48.74 | | +5.430 |
| 8 | Maud van Goor (Senior) | NED | 50.27 | SB | +6.960 |
| 9 | Wietske Rem (Neo) | NED | 51.53 | | +8.220 |
| 10 | Fimke Anna Giliam (Neo) | NED | 52.20 | SB | +8.890 |
| 11 | Jip Spel (Senior) | NED | 55.23 | | +11.920 |
| 12 | Marit de Jong (A1) | NED | 56.42 | SB | +13.110 |
| 13 | Nienke Markensteijn (Neo) | NED | 56.99 | SB | +13.680 |
| 14 | Annelotte Bex (Neo) | NED | 57.42 | | +14.110 |
| 15 | Anouk Goossens (Senior) | NED | 57.91 | PB | +14.600 |
| 16 | Adrianne de Roo (Neo) | NED | 1:01.87 | | +18.560 |
| 17 | Wendy Van Roosmalen (Neo) | NED | 1:05.73 | PB | +22.420 |
| 18 | Jose Ebbinge (Senior) | NED | 1:08.57 | | +25.260 |
| 19 | Lisa Mulder (Neo) | NED | 1:09.52 | SB | +26.210 |
| 20 | Rixt Hoekstra (Neo) | NED | 1:11.59 | SB | +28.280 |
| 21 | Dagmar Mul (Neo) | NED | 1:12.16 | PB | +28.850 |
| 22 | Irene Heijink (Neo) | NED | 1:13.09 | | +29.780 |
| 23 | Rianne Hazelhoff (Senior) | NED | 1:15.67 | SB | +32.360 |
| 24 | Lisanne van der Werf (Senior) | NED | 1:20.01 | | +36.700 |
| 25 | Lara van Ravenstein (Neo) | NED | 1:20.36 | | +37.050 |
| DNS | Anne de Jonge (Neo) | NED | DNS | | - |