| 1 | Renee Lauppe (B1) | NED | 45.22 | | - |
| 2 | Iris van Toorenburg (B2) | NED | 45.25 | | +0.030 |
| 3 | Laura Dreyer (B2) | NED | 45.87 | | +0.650 |
| 4 | Marléne Vermeij (B2) | NED | 46.04 | | +0.820 |
| 5 | Britt van den Boogert (B1) | NED | 46.87 | | +1.650 |
| 6 | Annabelle Neefjes (C2) | NED | 47.29 | | +2.070 |
| 7 | Merel van Toorenburg (C2) | NED | 47.59 | | +2.370 |
| 8 | Daniëlle Hoogendoorn (D2) | NED | 47.75 | | +2.530 |
| 9 | Miranda Slootweg (B1) | NED | 47.98 | | +2.760 |
| 10 | Remi Meeringa (B1) | NED | 48.21 | | +2.990 |
| 11 | Amanda Vernet (B2) | NED | 49.19 | | +3.970 |
| 12 | Kimberly Bosch (D1) | NED | 49.43 | | +4.210 |
| 13 | Lotte Fussenich (Neo) | NED | 49.71 | | +4.490 |
| 14 | Liona Vrancken (D2) | NED | 51.68 | | +6.460 |
| 15 | Jaccoline Zegers (C1) | NED | 52.26 | SB | +7.040 |
| 16 | Imke Janssen (D1) | NED | 53.00 | | +7.780 |
| 17 | Josje Zwartkruis (D2) | NED | 53.06 | | +7.840 |
| 18 | Jamie Verhoeven (C2) | NED | 53.39 | | +8.170 |
| 19 | Pam Berghuis (D1) | NED | 54.03 | | +8.810 |
| 20 | Manon Gremmen (D2) | NED | 54.40 | | +9.180 |
| 21 | Sabine Gnodde (D2) | NED | 54.81 | | +9.590 |
| 22 | Lisanne Bugter (E2) | NED | 56.00 | | +10.780 |
| 23 | Janne van den Elzen (D2) | NED | 59.47 | SB | +14.250 |
| 24 | Denise Schoonewil (D2) | NED | 1:00.52 | | +15.300 |
| 25 | Naomi Hoeks (D1) | NED | 1:02.45 | SB | +17.230 |
| 26 | Lonneke Gremmen (E2) | NED | 1:02.82 | | +17.600 |
| 27 | Florianne Kool (D2) | NED | 1:05.75 | | +20.530 |
| 28 | Nicolle van Rijswijk (D1) | NED | 1:06.27 | | +21.050 |
| 29 | Nynke Vredenbregt (D2) | NED | 1:09.41 | | +24.190 |
| 30 | Jara Stevens (E2) | NED | 1:12.17 | | +26.950 |