| 1 | Britt van de Put (D1) | NED | 44.98 | SB | - |
| 2 | Jolien van den Berg (Senior) | NED | 45.15 | SB | +0.170 |
| 3 | Nika van Dasselaar (C1) | NED | 45.44 | | +0.460 |
| 4 | Allessandra Primavera (B1) | NED | 45.47 | SB | +0.490 |
| 5 | Sofie Bouw (D1) | NED | 45.48 | SB | +0.500 |
| 6 | Frederieke Jansen (Neo) | NED | 45.93 | SB | +0.950 |
| 7 | Janna Bolsius (D1) | NED | 45.95 | SB | +0.970 |
| 8 | Esther van Leeuwen (Neo) | NED | 46.15 | | +1.170 |
| 9 | Lien van Dasselaar (D2) | NED | 46.38 | | +1.400 |
| 10 | Sophie Rijnders (A1) | NED | 46.81 | | +1.830 |
| 11 | Ine Bonthuis (B1) | NED | 47.08 | SB | +2.100 |
| 12 | Liotte Smits (B1) | NED | 47.21 | SB | +2.230 |
| 13 | Isa Leroy (E2) | NED | 47.35 | | +2.370 |
| 14 | Lauren Teunissen (D2) | NED | 47.54 | SB | +2.560 |
| 15 | Florien Bolks (B1) | NED | 47.76 | SB | +2.780 |
| 16 | Kim Leliveld (Senior) | NED | 47.89 | SB | +2.910 |
| 17 | Linda Eggenkamp (M45) | NED | 48.84 | SB | +3.860 |
| 18 | Julia de Vos (E2) | BRA | 49.56 | SB | +4.580 |
| 19 | Leoniek Anema (B1) | NED | 49.57 | SB | +4.590 |
| 20 | Emma Kole (D2) | NED | 50.47 | SB | +5.490 |
| 21 | Jorien Oosterwijk (M45) | NED | 50.64 | SB | +5.660 |
| 22 | Nienke Vinke (D2) | NED | 50.91 | SB | +5.930 |
| 23 | Isa Molenaar (D2) | NED | 51.04 | | +6.060 |
| 24 | Flore Room (C1) | NED | 51.14 | SB | +6.160 |
| 25 | Diede Wallet (D2) | NED | 51.21 | SB | +6.230 |
| 26 | Monique van Dorresteijn (B2) | NED | 51.41 | SB | +6.430 |
| 27 | Gaya Gritter (C2) | NED | 51.42 | SB | +6.440 |
| 28 | Kate Lynn Schouten (E1) | NED | 51.74 | | +6.760 |
| 29 | Demi Meinten (D1) | NED | 52.03 | SB | +7.050 |
| 30 | Senna Lamme (E1) | NED | 52.23 | | +7.250 |
| 31 | Norah Smits (C1) | NED | 52.86 | | +7.880 |
| 32 | Linde Rijnders (D2) | NED | 53.02 | SB | +8.040 |
| 33 | Luna van Polen (E2) | NED | 54.56 | SB | +9.580 |
| 34 | Maaike Koelewijn (E1) | NED | 55.09 | SB | +10.110 |
| 35 | Myrthe van der Grint (C1) | NED | 55.55 | SB | +10.570 |
| 36 | Vivianne Herder (D2) | NED | 57.48 | | +12.500 |
| 37 | Charmayne van den Berg (E1) | NED | 59.81 | SB | +14.830 |
| 38 | Jorinde van den Akker (E2) | NED | 1:01.43 | SB | +16.450 |
| 39 | Mijke Theunissen (C1) | NED | 1:02.45 | | +17.470 |
| 40 | Naomi Pols (E2) | NED | 1:02.78 | SB | +17.800 |
| 41 | Gesa Rüdiger (E2) | NED | 1:03.22 | | +18.240 |
| 42 | Demi Brouwer (F2) | NED | 1:04.06 | SB | +19.080 |
| 43 | Silke Kabel (F2) | NED | 1:04.09 | SB | +19.110 |
| 44 | Marit Jansma (D1) | NED | 1:09.44 | | +24.460 |
| 45 | Pastel Riemersma (E2) | NED | 1:12.55 | | +27.570 |
| 46 | Carlijn Kaaij (E2) | NED | 1:12.58 | | +27.600 |
| 47 | Pia Rudiger (F2) | NED | 1:13.64 | | +28.660 |
| 48 | Esther Rüdiger (F1) | NED | 1:17.28 | | +32.300 |
| DQ | Julia Lensink (B1) | NED | DQ | | - |