| 1 | Sugar Todd (C1) | USA | 43.89 | | - |
| 2 | Netanya Mintz (Neo) | USA | 44.10 | | +0.210 |
| 3 | Katrina Hancock (C1) | USA | 44.87 | | +0.980 |
| 4 | Alice Hagen (M30) | USA | 44.89 | | +1.000 |
| 5 | Lana Gehring (C1) | USA | 44.95 | | +1.060 |
| 6 | Mary Grace (D2) | USA | 44.97 | | +1.080 |
| 7 | Heidi Miller-Shockley (Senior) | USA | 46.32 | | +2.430 |
| 8 | Katie Brezovar (C1) | USA | 46.70 | | +2.810 |
| 9 | Deborah Movrich (Senior) | USA | 47.24 | | +3.350 |
| 10 | Stacie Bruening (C1) | USA | 48.33 | | +4.440 |
| 11 | Carla Langenthal (Senior) | USA | 48.88 | | +4.990 |
| 12 | Susan Vehe (C1) | USA | 50.71 | | +6.820 |
| 13 | Megan Foss (C1) | USA | 50.91 | | +7.020 |
| 14 | Paige Brehm (C1) | USA | 51.10 | | +7.210 |
| 15 | Heather Wickstrom (M30) | USA | 51.16 | | +7.270 |
| 16 | Hayley DeHaan (D2) | USA | 51.60 | | +7.710 |
| 17 | Abigail Nicks (n/a) | USA | 53.61 | | +9.720 |
| 18 | Joelle Wightman (n/a) | USA | 53.89 | | +10.000 |
| 19 | Natalie Ristau (C1) | USA | 54.08 | | +10.190 |
| 20 | Sonja Rani (n/a) | USA | 54.09 | | +10.200 |
| 21 | Cindi Hart (n/a) | USA | 54.77 | | +10.880 |
| 22 | Brenda Trolinger (Senior) | USA | 55.06 | | +11.170 |
| 23 | Lindsey Hagstrom (n/a) | USA | 55.68 | | +11.790 |
| 24 | Brooke Gall (D1) | USA | 56.28 | SB | +12.390 |
| 25 | Sophie Ristau (D1) | USA | 57.89 | | +14.000 |
| 26 | Virginia Wightman (n/a) | USA | 1:01.50 | | +17.610 |
| 27 | Michelle Callahan (n/a) | USA | 1:05.00 | | +21.110 |
| 28 | Madison Hart (n/a) | USA | 1:06.82 | | +22.930 |
| 29 | Amanda Hoopingarner (n/a) | USA | 1:48.62 | PB | +1.04.730 |