| 1 | Nina van Tol (B1) | NED | 42.66 | | - |
| 2 | Julia Berentschot (B1) | NED | 42.83 | | +0.170 |
| 3 | Daniëlle Vrolijk (B2) | NED | 44.27 | PB | +1.610 |
| 4 | Manouk `t Mannetje (Senior) | NED | 44.37 | | +1.710 |
| 5 | Catja Rotgans (B1) | NED | 45.29 | | +2.630 |
| 6 | Merith van 't Hoff (B2) | NED | 45.96 | | +3.300 |
| 7 | Eveline de Groot (B2) | NED | 46.10 | | +3.440 |
| 8 | Patricia van der Kooy (A1) | NED | 46.23 | PB | +3.570 |
| 9 | Charlotte Kors (B2) | NED | 46.40 | | +3.740 |
| 10 | Daisy van Dijk (Neo) | NED | 46.80 | | +4.140 |
| 11 | Nicole van der Helm (C2) | NED | 46.83 | | +4.170 |
| 12 | Miranda Slootweg (B1) | NED | 47.34 | | +4.680 |
| 13 | Evelien van Tol (A1) | NED | 47.95 | | +5.290 |
| 14 | Annelies Oude Alink (B2) | NED | 48.06 | SB | +5.400 |
| 15 | Mariëlle Koek (Senior) | NED | 48.13 | SB | +5.470 |
| 16 | Marianne de Neeling (M50) | NED | 48.28 | | +5.620 |
| 17 | Loys Vermeijden (B2) | NED | 48.55 | PB | +5.890 |
| 18 | Dominique Lengkeek (C2) | NED | 49.05 | SB | +6.390 |
| 19 | Kira Abbenhuijs (C2) | NED | 49.43 | SB | +6.770 |
| 20 | Feia Vermeyden (C2) | NED | 50.60 | SB | +7.940 |
| 21 | Sabine van der Kooy (C2) | NED | 50.68 | | +8.020 |
| 22 | Desiree van Reeuwijk (C2) | NED | 51.02 | | +8.360 |
| 23 | Joyce Buijs (B1) | NED | 51.26 | | +8.600 |
| 24 | Christel Dekker (Neo) | NED | 52.04 | SB | +9.380 |
| 25 | Evelien Kors (C1) | NED | 52.90 | | +10.240 |
| 26 | Mandy Duynisveld (C1) | NED | 52.97 | | +10.310 |
| 27 | Linda Milatz (C1) | NED | 53.11 | | +10.450 |
| 28 | Dita Adema (C1) | NED | 54.27 | | +11.610 |
| 29 | Manon Spruit (D2) | NED | 54.50 | | +11.840 |
| 30 | Gera Zonneveld (E2) | NED | 1:00.52 | | +17.860 |
| 31 | Lisa Ann van Steenbergen (D1) | NED | 1:01.93 | SB | +19.270 |
| 32 | Joanna Janssen (D2) | NED | 1:05.81 | | +23.150 |
| DNS | Claudia Oranje (B2) | NED | DNS | | - |