| 1 | Bernadette Ramaker (A1) | NED | 44.99 | | - |
| 2 | Patricia McKay (B2) | NED | 45.80 | | +0.810 |
| 3 | Jolanda Grimbergen (M40) | NED | 46.74 | SB | +1.750 |
| 4 | Meike Hoogeboom (Neo) | NED | 49.09 | | +4.100 |
| 5 | Jeske Hoogeboom (C2) | NED | 49.36 | | +4.370 |
| 6 | Jip van Elst (D2) | NED | 51.16 | SB | +6.170 |
| 7 | Elianne Roorda (B2) | NED | 51.34 | | +6.350 |
| 8 | Yael van Egmond (C1) | NED | 52.53 | | +7.540 |
| 9 | Julia Faber (C2) | NED | 54.13 | PB | +9.140 |
| 10 | Simone van der Hulst (Neo) | NED | 54.16 | PB | +9.170 |
| 11 | Dagmar de Bruin (M30) | NED | 54.21 | | +9.220 |
| 12 | Marije Kea (C2) | NED | 54.78 | PB | +9.790 |
| 13 | Karin Sneekes-van Essen (M40) | NED | 55.52 | SB | +10.530 |
| 14 | Linda van Wel (Neo) | NED | 57.07 | SB | +12.080 |
| 15 | Marlies Borst (B2) | NED | 58.59 | | +13.600 |
| 16 | Joyce Kuijper (C1) | NED | 1:00.36 | | +15.370 |
| 17 | Roswitha van der Kooy (M45) | NED | 1:02.04 | PB | +17.050 |
| 18 | Marloes Tesselaar (Neo) | NED | 1:02.38 | SB | +17.390 |
| 19 | Majsa Storbeck (C1) | NED | 1:02.75 | PB | +17.760 |
| 20 | Chayenne Kager (D2) | NED | 1:03.28 | | +18.290 |
| 21 | Tessa.00 Duivenvoorden (E1) | NED | 1:03.86 | SB | +18.870 |
| 22 | Laura Duivenvoorden (E2) | NED | 1:04.70 | | +19.710 |
| 23 | Annemarie Hotzes (C2) | NED | 1:04.84 | | +19.850 |
| 24 | Anita Wever-Pielaet (M40) | NED | 1:05.02 | | +20.030 |
| 25 | Myrthe van Dok (F2) | NED | 1:06.02 | SB | +21.030 |
| 26 | Zita den Das (E2) | NED | 1:07.75 | PB | +22.760 |
| 27 | Mirte Bos (D2) | NED | 1:08.20 | | +23.210 |
| 28 | Fiep Kleine (D1) | NED | 1:08.89 | | +23.900 |
| 29 | Irma Portegijs (D1) | NED | 1:09.21 | SB | +24.220 |
| 30 | Richtje Taal (M60) | NED | 1:10.26 | | +25.270 |
| 31 | Britt Klok (E1) | NED | 1:10.61 | | +25.620 |
| 32 | Hermieke Veldman (M45) | NED | 1:10.63 | SB | +25.640 |
| 33 | Fleur Hopman (E2) | NED | 1:14.43 | SB | +29.440 |
| 34 | Bonnie Berkeveld (C1) | NED | 1:14.71 | | +29.720 |