| 1 | Eva Riemersma-van Rheenen (M30) | NED | 45.96 | SB | - |
| 2 | Anna Wester (A1) | NED | 46.46 | | +0.506 |
| 3 | Shirley Jaarsma (M30) | NED | 46.76 | | +0.806 |
| 4 | Tjalda van Grieken (Neo) | NED | 47.27 | SB | +1.310 |
| 5 | Nienke Boekestijn (A2) | NED | 47.68 | SB | +1.724 |
| 6 | Maaike van Zijll (Neo) | NED | 50.12 | | +4.159 |
| 7 | Angelique van Rijn (Senior) | NED | 52.63 | SB | +6.673 |
| 8 | Eline Beemsterboer (M45) | NED | 54.27 | SB | +8.313 |
| 9 | Hanneke te Giffel (Senior) | NED | 57.83 | PB | +11.874 |
| 10 | Marian Bosch-Rekveldt (M40) | NED | 58.05 | SB | +12.094 |
| 11 | Carolien van Geest (Senior) | NED | 58.72 | SB | +12.768 |
| 12 | Vivianne de Vries (Senior) | NED | 59.80 | SB | +13.840 |
| 13 | Roos Weber (M45) | NED | 1:01.78 | SB | +15.828 |
| 14 | Diane Bacco-Geldof (Senior) | NED | 1:03.73 | SB | +17.776 |
| 15 | Monique de Graaf (M50) | NED | 1:05.13 | PB | +19.178 |