| 1 | Sanne Veenboer (Senior) | NED | 45.58 | SB | - |
| 2 | Naomi Kroon (C1) | NED | 46.76 | | +1.180 |
| 3 | Renske Kramer (Senior) | NED | 47.08 | SB | +1.500 |
| 4 | Clarissa Hartholt (D1) | NED | 48.10 | | +2.520 |
| 5 | Sam Veltum (A2) | NED | 48.34 | PB | +2.760 |
| 6 | Sara Pruiksma (C1) | NED | 49.18 | | +3.600 |
| 7 | Ynethe Zutt (C1) | NED | 49.82 | | +4.240 |
| 8 | Tessa Ankersmit (C2) | NED | 50.53 | PB | +4.950 |
| 9 | Fleur Gielink (D2) | NED | 50.71 | | +5.130 |
| 10 | Dewi Kroon (D1) | NED | 50.74 | | +5.160 |
| 11 | Lexie Ruiter (E2) | NED | 50.83 | | +5.250 |
| 12 | Anniek van de Kreeke (C1) | NED | 51.27 | | +5.690 |
| 13 | Benthe Sjoerds (C1) | NED | 51.36 | | +5.780 |
| 14 | Jonna Molhoek (D1) | NED | 52.05 | PB | +6.470 |
| 15 | Rielle Hageman (A1) | NED | 53.25 | | +7.670 |
| 16 | Lorna Bosma (D2) | NED | 53.41 | PB | +7.830 |
| 17 | Tamara van Kroonenburg (D2) | NED | 54.87 | | +9.290 |
| 18 | Sophie Ankersmit (E2) | NED | 54.98 | | +9.400 |
| 19 | Myrthe van der Putten (D1) | NED | 58.34 | PB | +12.760 |
| 20 | Elin Biersteker (B1) | NED | 1:01.84 | | +16.260 |
| 21 | Quinty Mooij (D2) | NED | 1:02.02 | PB | +16.440 |
| 22 | June van Gellekom (F1) | NED | 1:04.63 | PB | +19.050 |
| 23 | Tess Ruiter (F2) | NED | 1:05.00 | PB | +19.420 |
| 24 | Lizz Steltenpool (F2) | NED | 1:08.43 | | +22.850 |