| 1 | Chantal Hendriks (Senior) | NED | 45.42 | | - |
| 2 | Gwen Konter (B2) | NED | 45.57 | | +0.150 |
| 3 | Julia de Vos (C2) | BRA | 46.76 | SB | +1.340 |
| 4 | Quinty van Klink (Neo) | NED | 46.95 | | +1.530 |
| 5 | Emma van der Lied (B2) | NED | 49.00 | | +3.580 |
| 6 | Joelle Winkel (Neo) | NED | 49.71 | SB | +4.290 |
| 7 | Bryndis van Schijndel (B1) | NED | 49.93 | | +4.510 |
| 8 | Kaija Berendschot (Neo) | NED | 50.79 | SB | +5.370 |
| 9 | Alina van de Kracht (Neo) | NED | 53.10 | SB | +7.680 |
| 10 | Floor van Schijndel (C1) | NED | 53.42 | SB | +8.000 |
| 11 | Ciska Koole (M45) | NED | 54.06 | SB | +8.640 |
| 12 | Paula Teeuwen (Neo) | NED | 56.19 | SB | +10.770 |
| 13 | Anne Maaike Van Aken (Neo) | NED | 1:08.98 | SB | +23.560 |