| 1 | Nienke Kooistra (A1) | NED | 44.26 | SB | - |
| 2 | Elsbeth Bruinsma (Senior) | NED | 44.51 | | +0.250 |
| 3 | Carolien Hunneman (Neo) | NED | 45.38 | | +1.120 |
| 4 | Jolijn de Vries (Senior) | NED | 45.62 | | +1.360 |
| 5 | Jildou van Solkema (A2) | NED | 45.86 | | +1.600 |
| 6 | Marije Reitsma (A1) | NED | 46.54 | SB | +2.280 |
| 7 | Roelien Donker (Senior) | NED | 46.69 | | +2.430 |
| 8 | Chanou de Roos (Neo) | NED | 47.57 | | +3.310 |
| 9 | Janette de Groot-Bron (M30) | NED | 47.64 | | +3.380 |
| 10 | Sandra Zijlstra (Senior) | NED | 47.89 | | +3.630 |
| 11 | Mettie de Vegt (Neo) | NED | 47.93 | | +3.670 |
| 12 | Jildou Leemburg (Senior) | NED | 48.08 | SB | +3.820 |
| 13 | Deborah van der Valk (Neo) | NED | 49.00 | | +4.740 |
| 14 | Leonie Bezema (Neo) | NED | 49.27 | | +5.010 |
| 15 | Thea Kroontje (M60) | NED | 49.79 | | +5.530 |
| 16 | Jildau van der Weij (Neo) | NED | 49.96 | | +5.700 |
| 17 | Anneke Fransman-Balster (M55) | NED | 54.14 | | +9.880 |
| DQ | Leslie Koen (A2) | NED | DQ | | - |