| 1 | Sylke Kas (B2) | NED | 42.04 | SB | - |
| 2 | Romy de Jong (A1) | NED | 42.37 | | +0.330 |
| 3 | Rosalie van Vliet (D2) | NED | 44.00 | | +1.960 |
| 4 | Gerdien Meijer-Verweij (M50) | NED | 44.19 | | +2.150 |
| 5 | Femke de Boer.02 (B1) | NED | 45.32 | | +3.280 |
| 6 | Melissa Wenneker (C2) | NED | 45.58 | | +3.540 |
| 7 | Erica Della Guardia-de Jong (M45) | NED | 46.72 | | +4.680 |
| 8 | Jasmijn Soet (C2) | NED | 46.94 | | +4.900 |
| 9 | Fleur Brink (C2) | NED | 47.00 | | +4.960 |
| 10 | Femke Berends (B2) | NED | 47.31 | | +5.270 |
| 11 | Roos Schieman (C1) | NED | 47.59 | | +5.550 |
| 12 | Eveline Hermsen (A2) | NED | 47.65 | SB | +5.610 |
| 13 | Maika Schouten (A2) | NED | 49.39 | | +7.350 |
| 14 | Donna Hijkoop (B1) | NED | 49.88 | SB | +7.840 |
| 15 | Sanne Strijker (C2) | NED | 49.97 | PB | +7.930 |
| 16 | Kathleen Thomaes (Senior) | BEL | 51.09 | PB | +9.050 |
| 17 | Luka Beers (B1) | NED | 52.12 | SB | +10.080 |
| 18 | Ilse Bierma (D1) | NED | 52.45 | | +10.410 |
| 19 | Vera Vinje (D2) | NED | 52.48 | | +10.440 |
| 20 | Yasmin Kaldenbach (C2) | NED | 52.51 | | +10.470 |
| 21 | Lara Kaldenbach (D2) | NED | 52.99 | | +10.950 |
| 22 | Jola Vocking (B2) | NED | 53.43 | SB | +11.390 |
| 23 | José van Beek (M55) | NED | 54.07 | SB | +12.030 |
| 24 | Anna Sijbrandij (D1) | NED | 54.92 | | +12.880 |
| 25 | Laura Salomons (B1) | NED | 55.21 | SB | +13.170 |
| 26 | Iris Engel (A1) | NED | 55.33 | | +13.290 |
| 27 | Esmée Meijer (D1) | NED | 55.63 | | +13.590 |
| 28 | Cindy van Wijnbergen (E2) | NED | 1:01.22 | | +19.180 |
| 29 | Ayla Kaldenbach (F2) | NED | 1:11.12 | | +29.080 |
| 30 | Meike Paay (E2) | NED | 1:14.07 | SB | +32.030 |
| DNS | Chong Pei (Neo) | CHN | DNS | | - |
| DNS | Jiaying Tao (Senior) | CHN | DNS | | - |
| DNS | Dan Li (Senior) | CHN | DNS | | - |
| DNS | Qi Yin (Senior) | CHN | DNS | | - |
| DNF | Astrid Janssen (M55) | NED | DNF | | - |
| DNS | Darya Rashid (A1) | NED | DNS | | - |
| DQ | Sanne.00 de Vries (A2) | NED | DQ | | - |