| 1 | Lotte Elfferich (Neo) | NED | 45.15 | | - |
| 2 | Fedor Tillie (Neo) | NED | 45.44 | | +0.290 |
| 3 | Anna Wester (Neo) | NED | 46.31 | | +1.160 |
| 4 | Annelies Oude Alink (Senior) | NED | 46.48 | SB | +1.330 |
| 5 | Jolijn van Leeuwen (Senior) | NED | 48.54 | SB | +3.390 |
| 6 | Lysbet de Boer (Senior) | NED | 48.60 | SB | +3.450 |
| 7 | Iris Gimpel (Senior) | NED | 48.74 | | +3.590 |
| 8 | Lisa van der Veen (M35) | NED | 48.75 | SB | +3.600 |
| 9 | Kim Hoekstra (B2) | NED | 48.81 | SB | +3.660 |
| 10 | Puk Koole (A2) | NED | 48.92 | SB | +3.770 |
| 11 | Anne-Linde Paans (Neo) | NED | 50.00 | | +4.850 |
| 12 | Yara van Wieringen (Neo) | NED | 50.68 | | +5.530 |
| 13 | Yvonne van der Voort (M40) | NED | 52.00 | SB | +6.850 |
| 14 | Max Baneke (Neo) | NED | 53.04 | PB | +7.890 |
| 15 | Josta Bosma (Senior) | NED | 54.57 | SB | +9.420 |
| 16 | Niels Ruijter (Neo) | NED | 54.91 | | +9.760 |
| 17 | Annika Tol (Senior) | NED | 54.94 | SB | +9.790 |
| 18 | Astrid Fischer (Senior) | NED | 55.50 | SB | +10.350 |
| 19 | Claudia Beemster (M45) | NED | 55.91 | | +10.760 |
| 20 | Ilze Lutz (M35) | NED | 57.00 | | +11.850 |
| 21 | Laura Van Melle (Senior) | NED | 58.75 | PB | +13.600 |
| 22 | Maya Mokasay (Senior) | NED | 58.91 | | +13.760 |
| 23 | Wieke Heideman (M30) | NED | 58.92 | SB | +13.770 |
| 24 | Marieke Franssen (M35) | NED | 58.98 | SB | +13.830 |
| 25 | Kristien Vogel (Neo) | NED | 59.21 | PB | +14.060 |
| 26 | Isa Baas (B1) | NED | 1:01.45 | PB | +16.300 |
| 27 | Tanneke van't Westeinde (M45) | NED | 1:01.77 | SB | +16.620 |
| 28 | Gesina Tuinier (Senior) | NED | 1:02.15 | | +17.000 |
| 29 | Kimberly Vuurboom (Senior) | NED | 1:03.53 | SB | +18.380 |
| 30 | Joanna Blaisse (M65) | NED | 1:03.93 | | +18.780 |
| 31 | Bauke De Haan (Neo) | NED | 1:04.28 | PB | +19.130 |
| 32 | Anne-Geerte Beetsma (M30) | NED | 1:05.66 | SB | +20.510 |
| 33 | Lieuwe Olthof (Neo) | NED | 1:06.35 | PB | +21.200 |
| 34 | Nienke Nijdam (Neo) | NED | 1:06.36 | SB | +21.210 |
| 35 | Thijs Kuijpers (Neo) | NED | 1:16.06 | PB | +30.910 |
| 36 | Jan Garritzen (Neo) | NED | 1:16.41 | SB | +31.260 |
| DNS | Josefien Van Der Laan (Neo) | NED | DNS | | - |